Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells.

ABSTRACT: Recombinant mouse strains that harbor tightly controlled transgene expression proved to be indispensible tools to elucidate gene function. Different strategies have been employed to achieve controlled induction of the transgene. However, many models are accompanied by a considerable level of basal expression in the non-induced state. Thereby, applications that request tight control of transgene expression, such as the expression of toxic genes and the investigation of immune response to neo antigens are excluded. We developed a new Cre/loxP-based strategy to achieve strict control of transgene expression. This strategy was combined with RMCE (recombinase mediated cassette exchange) that facilitates the targeting of genes into a tagged site in ES cells. The tightness of regulation was confi

SUBMITTER: Sandhu U 

PROVIDER: S-EPMC3017619 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature