Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.

ABSTRACT: Identifying low-abundance mutations within wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. However, utilizing the clinical and diagnostic potential of rare mutations is limited by sensitivity of the molecular techniques employed, especially when the type and position of mutations are unknown. We have developed a novel platform that incorporates a synthetic reference sequence within a polymerase chain reaction (PCR) reaction, designed to enhance amplification of unknown mutant sequences during COLD-PCR (CO-amplification at Lower Denaturation temperature). This new platform enables an Improved and Complete Enrichment (ice-COLD-PCR) for all mutation types and eliminates shortcomings of previous formats of COLD-PCR. We eva

SUBMITTER: Milbury CA 

PROVIDER: S-EPMC3017621 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature