Reduction in hexokinase II levels results in decreased cardiac function and altered remodeling after ischemia/reperfusion injury.

ABSTRACT:

Rationale

Cardiomyocytes switch substrate utilization from fatty acid to glucose under ischemic conditions; however, it is unknown how perturbations in glycolytic enzymes affect cardiac response to ischemia/reperfusion (I/R). Hexokinase (HK)II is a HK isoform that is expressed in the heart and can bind to the mitochondrial outer membrane.

Objective

We sought to define how HKII and its binding to mitochondria play a role in cardiac response and remodeling after I/R.

Methods and results

We first showed that HKII levels and its binding to mitochondria are reduced 2 days after I/R. We then subjected the hearts of wild-type and heterozygote HKII knockout (HKII(+/)⁻) mice to I/R by coronary ligation. At baseline, HKII(+/)⁻ mice have normal cardiac function; however, they di

SUBMITTER: Wu R 

PROVIDER: S-EPMC3017633 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature