RISC RNA sequencing for context-specific identification of in vivo microRNA targets.

ABSTRACT:

Rationale

MicroRNAs (miRs) are expanding our understanding of cardiac disease and have the potential to transform cardiovascular therapeutics. One miR can target hundreds of individual mRNAs, but existing methodologies are not sufficient to accurately and comprehensively identify these mRNA targets in vivo.

Objective

To develop methods permitting identification of in vivo miR targets in an unbiased manner, using massively parallel sequencing of mouse cardiac transcriptomes in combination with sequencing of mRNA associated with mouse cardiac RNA-induced silencing complexes (RISCs).

Methods and results

We optimized techniques for expression profiling small amounts of RNA without introducing amplification bias and applied this to anti-Argonaute 2 immunoprecipitated RISCs

SUBMITTER: Matkovich SJ 

PROVIDER: S-EPMC3017647 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature