The two alpha-dox genes of Nicotiana attenuata: overlapping but distinct functions in development and stress responses.

ABSTRACT:

Background

Plant fatty acid alpha-dioxygenases (alpha-DOX) are oxylipin-forming enzymes induced by biotic and abiotic stresses, which also participate in developmental processes. In Nicotiana attenuata, herbivory strongly induces the expression of an alpha-dox1 gene. To determine its role, we silenced its expression using Agrobacterium-mediated plant transformation with an inverted repeat construct. More than half of the transformed lines showed a severe dwarf growth phenotype that was very similar to the phenotype of tomato plants mutated at a second alpha-dox isoform. This led us to identify the corresponding alpha-dox2 gene in N. attenuata and examine the regulation of both alpha-dox genes as well as the consequences of their silencing in plant development and anti-herbivore def

SUBMITTER: Steppuhn A 

PROVIDER: S-EPMC3017789 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature