High-throughput microarray technology in diagnostics of enterobacteria based on genome-wide probe selection and regression analysis.

ABSTRACT:

Background

The Enterobacteriaceae comprise a large number of clinically relevant species with several individual subspecies. Overlapping virulence-associated gene pools and the high overall genome plasticity often interferes with correct enterobacterial strain typing and risk assessment. Array technology offers a fast, reproducible and standardisable means for bacterial typing and thus provides many advantages for bacterial diagnostics, risk assessment and surveillance. The development of highly discriminative broad-range microbial diagnostic microarrays remains a challenge, because of marked genome plasticity of many bacterial pathogens.

Results

We developed a DNA microarray for strain typing and detection of major antimicrobial resistance genes of clinically relevant enter

SUBMITTER: Friedrich T 

PROVIDER: S-EPMC3017858 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature