A transcriptomic analysis of gene expression in the venom gland of the snake Bothrops alternatus (urutu).

ABSTRACT:

Background

The genus Bothrops is widespread throughout Central and South America and is the principal cause of snakebite in these regions. Transcriptomic and proteomic studies have examined the venom composition of several species in this genus, but many others remain to be studied. In this work, we used a transcriptomic approach to examine the venom gland genes of Bothrops alternatus, a clinically important species found in southeastern and southern Brazil, Uruguay, northern Argentina and eastern Paraguay.

Results

A cDNA library of 5,350 expressed sequence tags (ESTs) was produced and assembled into 838 contigs and 4512 singletons. BLAST searches of relevant databases showed 30% hits and 70% no-hits, with toxin-related transcripts accounting for 23% and 78% of the total tra

SUBMITTER: Cardoso KC 

PROVIDER: S-EPMC3017861 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature