A microbial detection array (MDA) for viral and bacterial detection.

ABSTRACT:

Background

Identifying the bacteria and viruses present in a complex sample is useful in disease diagnostics, product safety, environmental characterization, and research. Array-based methods have proven utility to detect in a single assay at a reasonable cost any microbe from the thousands that have been sequenced.

Methods

We designed a pan-Microbial Detection Array (MDA) to detect all known viruses (including phages), bacteria and plasmids and developed a novel statistical analysis method to identify mixtures of organisms from complex samples hybridized to the array. The array has broader coverage of bacterial and viral targets and is based on more recent sequence data and more probes per target than other microbial detection/discovery arrays in the literature. Family-spec

SUBMITTER: Gardner SN 

PROVIDER: S-EPMC3017867 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature