Comparative physical maps derived from BAC end sequences of tilapia (Oreochromis niloticus).

ABSTRACT:

Background

The Nile tilapia is the second most important fish in aquaculture. It is an excellent laboratory model, and is closely related to the African lake cichlids famous for their rapid rates of speciation. A suite of genomic resources has been developed for this species, including genetic maps and ESTs. Here we analyze BAC end-sequences to develop comparative physical maps, and estimate the number of genome rearrangements, between tilapia and other model fish species.

Results

We obtained sequence from one or both ends of 106,259 tilapia BACs. BLAST analysis against the genome assemblies of stickleback, medaka and pufferfish allowed identification of homologies for approximately 25,000 BACs for each species. We calculate that rearrangement breakpoints between tilapia and

SUBMITTER: Soler L 

PROVIDER: S-EPMC3018143 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature