System-level analysis of genes and functions affecting survival during nutrient starvation in Saccharomyces cerevisiae.

ABSTRACT: An essential property of all cells is the ability to exit from active cell division and persist in a quiescent state. For single-celled microbes this primarily occurs in response to nutrient deprivation. We studied the genetic requirements for survival of Saccharomyces cerevisiae when starved for either of two nutrients: phosphate or leucine. We measured the survival of nearly all nonessential haploid null yeast mutants in mixed populations using a quantitative sequencing method that estimates the abundance of each mutant on the basis of frequency of unique molecular barcodes. Starvation for phosphate results in a population half-life of 337 hr whereas starvation for leucine results in a half-life of 27.7 hr. To measure survival of individual mutants in each population we developed a stati

SUBMITTER: Gresham D 

PROVIDER: S-EPMC3018308 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature