Detection of respiratory viruses and the associated chemokine responses in serious acute respiratory illness.

ABSTRACT:

Background

A specific diagnosis of a lower respiratory viral infection is often difficult despite frequent clinical suspicion. This low diagnostic yield may be improved by use of sensitive detection methods and biomarkers.

Methods

The prevalence, clinical predictors and inflammatory mediator profile of respiratory viral infection in serious acute respiratory illness were investigated. Sequential bronchoalveolar lavage (BAL) fluids from all patients hospitalised with acute respiratory illness over 12 months (n=283) were tested for the presence of 17 respiratory viruses by multiplex PCR assay and for newly discovered respiratory viruses (bocavirus, WU and KI polyomaviruses) by single-target PCR. BAL samples also underwent conventional testing (direct immunoflorescence and vira

SUBMITTER: Sumino KC 

PROVIDER: S-EPMC3018337 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature