Kruppel-like factor-4 transcriptionally regulates VE-cadherin expression and endothelial barrier function.

ABSTRACT:

Rationale

Vascular endothelial (VE)-cadherin localized at adherens junctions (AJs) regulates endothelial barrier function. Because WNT (wingless) signaling-induced activation of the transcription factor Krüppel-like factor (KLF)4 may have an important role in mediating the expression of VE-cadherin and AJ integrity, we studied the function of KLF4 in regulating VE-cadherin expression and the control of endothelial barrier function.

Objective

The goal of this study was to determine the transcriptional role of KLF4 in regulating VE-cadherin expression and endothelial barrier function.

Methods and results

Expression analysis, microscopy, chromatin immunoprecipitation, electrophoretic mobility shift assays, and VE-cadherin-luciferase reporter experiments demonstrated that

SUBMITTER: Cowan CE 

PROVIDER: S-EPMC3018700 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature