Improved strategies for rapid identification of chemically cross-linked peptides using protein interaction reporter technology.

ABSTRACT: Protein interaction reporter (PIR) technology can enable identification of in vivo protein interactions with the use of specialized chemical cross-linkers, liquid chromatography, and high-resolution mass spectrometry. PIR-cross-linkers contain labile bonds that are specifically fragmented under low energy collision or photodissociation conditions in the mass spectrometer source, thus releasing cross-linked peptides. Successful analysis of PIR-cross-linked proteins requires the use of expected mathematical relationships between cross-linked complexes and released peptides after fragmentation of the labile PIR bonds. Presented here is a next-generation software tool, BLinks, for use in the analysis and identification of PIR-cross-linked proteins. BLinks is an advancement beyond our previous

SUBMITTER: Hoopmann MR 

PROVIDER: S-EPMC3018735 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature