Regulation of polar peptidoglycan biosynthesis by Wag31 phosphorylation in mycobacteria.

ABSTRACT:

Background

Sensing and responding to environmental changes is a central aspect of cell division regulation. Mycobacterium tuberculosis contains eleven Ser/Thr kinases, two of which, PknA and PknB, are key signaling molecules that regulate cell division/morphology. One substrate of these kinases is Wag31, and we previously showed that partial depletion of Wag31 caused morphological changes indicative of cell wall defects, and that the phosphorylation state of Wag31 affected cell growth in mycobacteria. In the present study, we further characterized the role of the Wag31 phosphorylation in polar peptidoglycan biosynthesis.

Results

We demonstrate that the differential growth among cells expressing different wag31 alleles (wild-type, phosphoablative, or phosphomimetic) is caused

SUBMITTER: Jani C 

PROVIDER: S-EPMC3019181 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature