High-sensitivity array analysis of gene expression for the early detection of disseminated breast tumor cells in peripheral blood.

ABSTRACT: Early detection is an effective means of reducing cancer mortality. Here, we describe a highly sensitive high-throughput screen that can identify panels of markers for the early detection of solid tumor cells disseminated in peripheral blood. The method is a two-step combination of differential display and high-sensitivity cDNA arrays. In a primary screen, differential display identified 170 candidate marker genes differentially expressed between breast tumor cells and normal breast epithelial cells. In a secondary screen, high-sensitivity arrays assessed expression levels of these genes in 48 blood samples, 22 from healthy volunteers and 26 from breast cancer patients. Cluster analysis identified a group of 12 genes that were elevated in the blood of cancer patients. Permutation analysis

SUBMITTER: Martin KJ 

PROVIDER: S-EPMC30192 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature