Gene discovery in the horned beetle Onthophagus taurus.

ABSTRACT:

Background

Horned beetles, in particular in the genus Onthophagus, are important models for studies on sexual selection, biological radiations, the origin of novel traits, developmental plasticity, biocontrol, conservation, and forensic biology. Despite their growing prominence as models for studying both basic and applied questions in biology, little genomic or transcriptomic data are available for this genus. We used massively parallel pyrosequencing (Roche 454-FLX platform) to produce a comprehensive EST dataset for the horned beetle Onthophagus taurus. To maximize sequence diversity, we pooled RNA extracted from a normalized library encompassing diverse developmental stages and both sexes.

Results

We used 454 pyrosequencing to sequence ESTs from all post-embryonic stages

SUBMITTER: Choi JH 

PROVIDER: S-EPMC3019233 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature