Comparison of transcriptomic landscapes of bovine embryos using RNA-Seq.

ABSTRACT:

Background

Advances in sequencing technologies have opened a new era of high throughput investigations. Although RNA-seq has been demonstrated in many organisms, no study has provided a comprehensive investigation of the bovine transcriptome using RNA-seq.

Results

In this study, we provide a deep survey of the bovine embryonic transcriptomes, the first application of RNA-seq in cattle. Embryos cultured in vitro were used as models to study early embryonic development in cattle. RNA amplified from limited amounts of starting total RNA were sequenced and mapped to the reference genome to obtain digital gene expression at single base resolution. In particular, gene expression estimates from more than 1.6 million unannotated bases in 1785 novel transcribed units were obtained. W

SUBMITTER: Huang W 

PROVIDER: S-EPMC3019235 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature