Sequential recruitment of SAGA and TFIID in a genomic response to DNA damage in Saccharomyces cerevisiae.

ABSTRACT: Eukaryotic genes respond to their environment by changing the expression of selected genes. The question we address here is whether distinct transcriptional responses to different environmental signals elicit distinct modes of assembly of the transcription machinery. In particular, we examine transcription complex assembly by the stress-directed SAGA complex versus the housekeeping assembly factor TFIID. We focus on genomic responses to the DNA damaging agent methyl methanesulfonate (MMS) in comparison to responses to acute heat shock, looking at changes in genome-wide factor occupancy measured by chromatin immunoprecipitation-microchip (ChIP-chip) and ChIP-sequencing analyses. Our data suggest that MMS-induced genes undergo transcription complex assembly sequentially, first involving SAGA

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC3019861 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature