Ablation of phospholamban and sarcolipin results in cardiac hypertrophy and decreased cardiac contractility.

ABSTRACT:

Aims

Improving the sarco(endo)plasmic reticulum (SR) Ca(2+)-ATPase (SERCA) function has clinical implications in treating heart failure. The present study aimed to determine the effect of constitutive activation of the SERCA pump on cardiac contractility in normal mice and during pressure-overload-induced cardiac hypertrophy.

Methods and results

The SERCA pump was constitutively activated in both atrial and ventricular chambers of the mouse heart by ablating its key regulators, phospholamban (PLN) and sarcolipin (SLN). The double-knockout (dKO) mice for PLN and SLN showed increased SERCA pump activity, Ca(2+) transients and SR Ca(2+) load, and developed cardiac hypertrophy. Echocardiographic measurements showed that the basal cardiac function was not affected in the young dK

SUBMITTER: Shanmugam M 

PROVIDER: S-EPMC3020129 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature