Quality control of the sheep bacterial artificial chromosome library, CHORI-243.

ABSTRACT:

Background

The sheep CHORI-243 bacterial artificial chromosome (BAC) library is being used in the construction of the virtual sheep genome, the sequencing and construction of the actual sheep genome assembly and as a source of DNA for regions of the genome of biological interest. The objective of our study is to assess the integrity of the clones and plates which make up the CHORI-243 library using the virtual sheep genome.

Findings

A series of analyses were undertaken based on the mapping the sheep BAC-end sequences (BESs) to the virtual sheep genome. Overall, very few plate specific biases were identified, with only three of the 528 plates in the library significantly affected. The analysis of the number of tail-to-tail (concordant) BACs on the plates identified a number o

SUBMITTER: Ratnakumar A 

PROVIDER: S-EPMC3020178 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature