Molecular detection, epidemiology, and genetic characterization of novel European field isolates of equine infectious anemia virus.

ABSTRACT: The application of molecular diagnostic techniques along with nucleotide sequence determination to permit contemporary phylogenetic analysis of European field isolates of equine infectious anemia virus (EIAV) has not been widely reported. As a result, of extensive testing instigated following the 2006 outbreak of equine infectious anemia in Italy, 24 farms with a history of exposure to this disease were included in this study. New PCR-based methods were developed, which, especially in the case of DNA preparations from peripheral blood cells, showed excellent correlation with OIE-approved agar gel immunodiffusion (AGID) tests for identifying EIAV-infected animals. In contrast, the OIE-recommended oligonucleotide primers for EIAV failed to react with any of the Italian isolates. Similar resu

SUBMITTER: Cappelli K 

PROVIDER: S-EPMC3020406 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature