Cultivation-independent analysis of bacteria in IDEXX Quanti-Tray/2000 fecal indicator assays.

ABSTRACT: Monitoring microbiological water quality is important for protecting water resources and the health of swimmers. Routine monitoring relies on cultivating fecal indicator bacteria (FIB), frequently using defined substrate technology. Defined substrate technology is designed to specifically enrich for FIB, but a complete understanding of the assay microbiology requires culture-independent analysis of the enrichments. This study aimed to identify bacteria in positive wells of Colilert and Enterolert Quanti-Tray/2000 (IDEXX Laboratories) FIB assays in environmental water samples and to quantify the degree of false-positive results for samples from an urban creek by molecular methods. Pooled Escherichia coli- and Enterococcus-positive Quanti-Tray/2000 enrichments, either from urban creek dry we

SUBMITTER: Sercu B 

PROVIDER: S-EPMC3020531 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature