H4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo.

ABSTRACT: While specific posttranslational modification patterns within the H3 and H4 tail domains are associated with the S-phase, their actual functions in replication-dependent chromatin assembly have not yet been defined. Here we used incorporation of trace amounts of recombinant proteins into naturally synchronous macroplasmodia of Physarum polycephalum to examine the function of H3 and H4 tail domains in replication-coupled chromatin assembly. We found that the H3/H4 complex lacking the H4 tail domain was not efficiently recovered in nuclei, whereas depletion of the H3 tail domain did not impede nuclear import but chromatin assembly failed. Furthermore, our results revealed that the proper pattern of acetylation on the H4 tail domain is required for nuclear import and chromatin assembly. This

SUBMITTER: Ejlassi-Lassallette A 

PROVIDER: S-EPMC3020919 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature