Transcription initiation patterns indicate divergent strategies for gene regulation at the chromatin level.

ABSTRACT: The application of deep sequencing to map 5' capped transcripts has confirmed the existence of at least two distinct promoter classes in metazoans: "focused" promoters with transcription start sites (TSSs) that occur in a narrowly defined genomic span and "dispersed" promoters with TSSs that are spread over a larger window. Previous studies have explored the presence of genomic features, such as CpG islands and sequence motifs, in these promoter classes, but virtually no studies have directly investigated the relationship with chromatin features. Here, we show that promoter classes are significantly differentiated by nucleosome organization and chromatin structure. Dispersed promoters display higher associations with well-positioned nucleosomes downstream of the TSS and a more clearly defi

SUBMITTER: Rach EA 

PROVIDER: S-EPMC3020932 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature