Multi-modal proteomic analysis of retinal protein expression alterations in a rat model of diabetic retinopathy.

ABSTRACT:

Background

As a leading cause of adult blindness, diabetic retinopathy is a prevalent and profound complication of diabetes. We have previously reported duration-dependent changes in retinal vascular permeability, apoptosis, and mRNA expression with diabetes in a rat model system. The aim of this study was to identify retinal proteomic alterations associated with functional dysregulation of the diabetic retina to better understand diabetic retinopathy pathogenesis and that could be used as surrogate endpoints in preclinical drug testing studies.

Methodology/principal findings

A multi-modal proteomic approach of antibody (Luminex)-, electrophoresis (DIGE)-, and LC-MS (iTRAQ)-based quantitation methods was used to maximize coverage of the retinal proteome. Transcriptomic profi

SUBMITTER: VanGuilder HD 

PROVIDER: S-EPMC3020973 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature