Ontology highlight
ABSTRACT:
SUBMITTER: Klein BJ
PROVIDER: S-EPMC3021056 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Klein Brianna J BJ Bose Daniel D Baker Kevin J KJ Yusoff Zahirah M ZM Zhang Xiaodong X Murakami Katsuhiko S KS
Proceedings of the National Academy of Sciences of the United States of America 20101227 2
Spt4/5 in archaea and eukaryote and its bacterial homolog NusG is the only elongation factor conserved in all three domains of life and plays many key roles in cotranscriptional regulation and in recruiting other factors to the elongating RNA polymerase. Here, we present the crystal structure of Spt4/5 as well as the structure of RNA polymerase-Spt4/5 complex using cryoelectron microscopy reconstruction and single particle analysis. The Spt4/5 binds in the middle of RNA polymerase claw and enclo ...[more]