Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity.

ABSTRACT: A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3' → 5' exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

SUBMITTER: Leung CH 

PROVIDER: S-EPMC3021625 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature