A key role for poly(ADP-ribose) polymerase 3 in ectodermal specification and neural crest development.

ABSTRACT:

Background

The PARP family member poly(ADP-ribose) polymerase 3 (PARP3) is structurally related to the well characterized PARP1 that orchestrates cellular responses to DNA strand breaks and cell death by the synthesis of poly(ADP-ribose). In contrast to PARP1 and PARP2, the functions of PARP3 are undefined. Here, we reveal critical functions for PARP3 during vertebrate development.

Principal findings

We have used several in vitro and in vivo approaches to examine the possible functions of PARP3 as a transcriptional regulator, a function suggested from its previously reported association with several Polycomb group (PcG) proteins. We demonstrate that PARP3 gene occupancy in the human neuroblastoma cell line SK-N-SH occurs preferentially with developmental genes regulating cel

SUBMITTER: Rouleau M 

PROVIDER: S-EPMC3022025 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature