Comprehensive polyadenylation site maps in yeast and human reveal pervasive alternative polyadenylation.

ABSTRACT: The emerging discoveries on the link between polyadenylation and disease states underline the need to fully characterize genome-wide polyadenylation states. Here, we report comprehensive maps of global polyadenylation events in human and yeast generated using refinements to the Direct RNA Sequencing technology. This direct approach provides a quantitative view of genome-wide polyadenylation states in a strand-specific manner and requires only attomole RNA quantities. The polyadenylation profiles revealed an abundance of unannotated polyadenylation sites, alternative polyadenylation patterns, and regulatory element-associated poly(A)(+) RNAs. We observed differences in sequence composition surrounding canonical and noncanonical human polyadenylation sites, suggesting novel noncoding RNA-spe

SUBMITTER: Ozsolak F 

PROVIDER: S-EPMC3022516 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature