Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells.

ABSTRACT:

Background

The Nucleotide Excision Repair (NER) pathway specialises in UV-induced DNA damage repair. Inherited defects in the NER can predispose individuals to Xeroderma Pigmentosum (XP). UV-induced DNA damage cannot account for the manifestation of XP in organ systems not directly exposed to sunlight. While the NER has recently been implicated in the repair of oxidative DNA lesions, it is not well characterised. Therefore we sought to investigate the role of NER factors Xeroderma Pigmentosum A (XPA), XPB and XPD in oxidative DNA damage-repair by subjecting lymphoblastoid cells from patients suffering from XP-A, XP-D and XP-B with Cockayne Syndrome to hydrogen peroxide (H2O2).

Results

Loss of functional XPB or XPD but not XPA led to enhanced sensitivity towards H2O2-induced

SUBMITTER: Gopalakrishnan K 

PROVIDER: S-EPMC3022891 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature