A molecular inversion probe assay for detecting alternative splicing.

ABSTRACT:

Background

A sensitive, high-throughput method for monitoring pre-mRNA splicing on a genomic scale is needed to understand the spectrum of alternatively spliced mRNA in human cells.

Results

We adapted Molecular Inversion Probes (MIPs), a padlock-probe based technology, for the multiplexed capture and quantitation of individual splice events in human tissues. Individual MIP capture probes can be quantified using either DNA microarrays or high-throughput sequencing, which permits independent assessment of each spliced junction. Using our methodology we successfully identified 100% of our positive controls and showed that there is a strong correlation between the data from our alternative splicing MIP (asMIP) assay and quantitative PCR.

Conclusion

The asMIP assay provide

SUBMITTER: Lin S 

PROVIDER: S-EPMC3022918 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature