New methods for next generation sequencing based microRNA expression profiling.

ABSTRACT:

Background

MicroRNAs are small non-coding RNA transcripts that regulate post-transcriptional gene expression. The millions of short sequence reads generated by next generation sequencing technologies make this technique explicitly suitable for profiling of known and novel microRNAs. A modification to the small-RNA expression kit (SREK, Ambion) library preparation method for the SOLiD sequencing platform is described to generate microRNA sequencing libraries that are compatible with the Illumina Genome Analyzer.

Results

High quality sequencing libraries can successfully be prepared from as little as 100 ng small RNA enriched RNA. An easy to use perl-based analysis pipeline called E-miR was developed to handle the sequencing data in several automated steps including data forma

SUBMITTER: Buermans HP 

PROVIDER: S-EPMC3022920 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature