Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array.

ABSTRACT:

Background

Recent progress in high-throughput technologies has greatly contributed to the development of DNA methylation profiling. Although there are several reports that describe methylome detection of whole genome bisulfite sequencing, the high cost and heavy demand on bioinformatics analysis prevents its extensive application. Thus, current strategies for the study of mammalian DNA methylomes is still based primarily on genome-wide methylated DNA enrichment combined with DNA microarray detection or sequencing. Methylated DNA enrichment is a key step in a microarray based genome-wide methylation profiling study, and even for future high-throughput sequencing based methylome analysis.

Results

In order to evaluate the sensitivity and accuracy of methylated DNA enrichment, w

SUBMITTER: Yang L 

PROVIDER: S-EPMC3023747 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature