An iterative workflow for mining the human intestinal metaproteome.

ABSTRACT:

Background

Peptide spectrum matching (PSM) is the standard method in shotgun proteomics data analysis. It relies on the availability of an accurate and complete sample proteome that is used to make interpretation of the spectra feasible. Although this procedure has proven to be effective in many proteomics studies, the approach has limitations when applied on complex samples of microbial communities, such as those found in the human intestinal tract. Metagenome studies have indicated that the human intestinal microbiome contains over 100 times more genes than the human genome and it has been estimated that this ecosystem contains over 5000 bacterial species. The genomes of the vast majority of these species have not yet been sequenced and hence their proteomes remain unknown. To en

SUBMITTER: Rooijers K 

PROVIDER: S-EPMC3023752 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature