Targeted protein depletion in Saccharomyces cerevisiae by activation of a bidirectional degron.

ABSTRACT:

Background

Tools for in vivo manipulation of protein abundance or activity are highly beneficial for life science research. Protein stability can be efficiently controlled by conditional degrons, which induce target protein degradation at restrictive conditions.

Results

We used the yeast Saccharomyces cerevisiae for development of a conditional, bidirectional degron to control protein stability, which can be fused to the target protein N-terminally, C-terminally or placed internally. Activation of the degron is achieved by cleavage with the tobacco etch virus (TEV) protease, resulting in quick proteolysis of the target protein. We found similar degradation rates of soluble substrates using destabilization by the N- or C-degron. C-terminal tagging of essential yeast proteins

SUBMITTER: Jungbluth M 

PROVIDER: S-EPMC3024245 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature