Optimizing optical flow cytometry for cell volume-based sorting and analysis.

ABSTRACT: Cell size is a defining characteristic central to cell function and ultimately to tissue architecture. The ability to sort cell subpopulations of different sizes would facilitate investigation at genomic and proteomic levels of mechanisms by which cells attain and maintain their size. Currently available cell sorters, however, cannot directly measure cell volume electronically, and it would therefore be desirable to know which of the optical measurements that can be made in such instruments provide the best estimate of volume. We investigated several different light scattering and fluorescence measurements in several different cell lines, sorting cell fractions from the high and low end of distributions, and measuring volume electronically to determine which sorting strategy yielded the be

SUBMITTER: Tzur A 

PROVIDER: S-EPMC3024321 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature