The development of metabolomic sampling procedures for Pichia pastoris, and baseline metabolome data.

ABSTRACT: Metabolic profiling is increasingly being used to investigate a diverse range of biological questions. Due to the rapid turnover of intracellular metabolites it is important to have reliable, reproducible techniques for sampling and sample treatment. Through the use of non-targeted analytical techniques such as NMR and GC-MS we have performed a comprehensive quantitative investigation of sampling techniques for Pichia pastoris. It was clear that quenching metabolism using solutions based on the standard cold methanol protocol caused some metabolite losses from P. pastoris cells. However, these were at a low level, with the NMR results indicating metabolite increases in the quenching solution below 5% of their intracellular level for 75% of metabolites identified; while the GC-MS results su

SUBMITTER: Tredwell GD 

PROVIDER: S-EPMC3025026 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature