Co-regulation of alternative splicing by diverse splicing factors in Caenorhabditis elegans.

ABSTRACT: Regulation of alternative splicing is controlled by pre-mRNA sequences (cis-elements) and trans-acting protein factors that bind them. The combinatorial interactions of multiple protein factors with the cis-elements surrounding a given alternative splicing event lead to an integrated splicing decision. The mechanism of multifactorial splicing regulation is poorly understood. Using a splicing-sensitive DNA microarray, we assayed 352 Caenorhabditis elegans alternative cassette exons for changes in embryonic splicing patterns between wild-type and 12 different strains carrying mutations in a splicing factor. We identified many alternative splicing events that are regulated by multiple splicing factors. Many splicing factors have the ability to behave as splicing repressors for some alternativ

SUBMITTER: Barberan-Soler S 

PROVIDER: S-EPMC3025544 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature