A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.

ABSTRACT: The Sox6 transcription factor plays critical roles in various cell types, including erythroid cells. Sox6-deficient mice are anemic due to impaired red cell maturation and show inappropriate globin gene expression in definitive erythrocytes. To identify new Sox6 target genes in erythroid cells, we used the known repressive double Sox6 consensus within the εy-globin promoter to perform a bioinformatic genome-wide search for similar, evolutionarily conserved motifs located within genes whose expression changes during erythropoiesis. We found a highly conserved Sox6 consensus within the Sox6 human gene promoter itself. This sequence is bound by Sox6 in vitro and in vivo, and mediates transcriptional repression in transient transfections in human erythroleukemic K562 cells and in primary eryth

SUBMITTER: Cantu' C 

PROVIDER: S-EPMC3025548 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature