Bacterial biota in reflux esophagitis and Barrett's esophagus.

ABSTRACT:

Aim

To identify the bacterial flora in conditions such as Barrettos esophagus and reflux esophagitis to determine if they are similar to normal esophageal flora.

Methods

Using broad-range 16S rDNA PCR, esophageal biopsies were examined from 24 patients [9 with normal esophageal mucosa, 12 with gastroesophageal reflux disease (GERD), and 3 with Barrettos esophagus]. Two separate broad-range PCR reactions were performed for each patient, and the resulting products were cloned. In one patient with Barrettos esophagus, 99 PCR clones were analyzed.

Results

Two separate clones were recovered from each patient (total = 48), representing 24 different species, with 14 species homologous to known bacteria, 5 homologous to unidentified bacteria, and 5 were not homologous (<97% i

SUBMITTER: Pei Z 

PROVIDER: S-EPMC3025696 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature