Influence of insulators on transgene expression from integrating and non-integrating lentiviral vectors.

ABSTRACT:

Background

The efficacy and biosafety of lentiviral gene transfer is influenced by the design of the vector. To this end, properties of lentiviral vectors can be modified by using cis-acting elements such as the modification of the U3 region of the LTR, the incorporation of the central flap (cPPT-CTS) element, or post-transcriptional regulatory elements such as the woodchuck post-transcriptional regulatory element (WPRE). Recently, several studies evaluated the influence of the incorporation of insulators into the integrating lentiviral vector genome on transgene expression level and position effects.

Methods

In the present study, the influence of the matrix attachment region (MAR) of the mouse immunoglobulin-κ (Ig-κ) or the chicken lysozyme (ChL) gene was studied on three t

SUBMITTER: Grandchamp N 

PROVIDER: S-EPMC3025823 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature