A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.

ABSTRACT:

Background

Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium Corynebacterium pseudotuberculosis, the etiological agent of caseous lymphadenitis (CLA) in sheep and goats.

Results

An optimized protocol of three-phase partitioning (TPP) was used to obtain the C. pseudotuberculosis exoproteins, and a newly introduced method of data-independent MS acquisition (LC-MSE) was employed for protein identification and label-free quantification. Additionally, the recently developed tool SurfG+ was used for in silico prediction of sub-cellular localization of the identified proteins. In total, 93 different extracellular proteins of C. p

SUBMITTER: Pacheco LG 

PROVIDER: S-EPMC3025830 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature