Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae.

ABSTRACT:

Background

The development of novel yeast strains with increased tolerance toward inhibitors in lignocellulosic hydrolysates is highly desirable for the production of bio-ethanol. Weak organic acids such as acetic and formic acids are necessarily released during the pretreatment (i.e. solubilization and hydrolysis) of lignocelluloses, which negatively affect microbial growth and ethanol production. However, since the mode of toxicity is complicated, genetic engineering strategies addressing yeast tolerance to weak organic acids have been rare. Thus, enhanced basic research is expected to identify target genes for improved weak acid tolerance.

Results

In this study, the effect of acetic acid on xylose fermentation was analyzed by examining metabolite profiles in a recombinant

SUBMITTER: Hasunuma T 

PROVIDER: S-EPMC3025834 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature