Toward understanding the mechanism of action of the yeast multidrug resistance transporter Pdr5p: a molecular modeling study.

ABSTRACT: Pleotropic drug resistant protein 5 (Pdr5p) is a plasma membrane ATP-binding cassette (ABC) transporter and the major drug efflux pump in Saccharomyces cerevisiae. The Pdr5p family of fungal transporters possesses a number of structural features significantly different from other modeled or crystallized ABC transporters, which include a reverse topology, an atypical ATP-binding site, a very low sequence similarity in the transmembrane section and long linkers between domains. These features present a considerable hurdle in molecular modeling studies of these important transporters. Here, we report the creation of an atomic model of Pdr5p based on a combination of homology modeling and ab initio methods, incorporating information from consensus transmembrane segment prediction, residue lipo

SUBMITTER: Rutledge RM 

PROVIDER: S-EPMC3026082 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature