Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells.

ABSTRACT: (13)C metabolic flux analysis (MFA) is the most comprehensive means of characterizing cellular metabolic states. Uniquely labeled isotopic tracers enable more focused analyses to probe specific reactions within the network. As a result, the choice of tracer largely determines the precision with which one can estimate metabolic fluxes, especially in complex mammalian systems that require multiple substrates. Here we have experimentally determined metabolic fluxes in a tumor cell line, successfully recapitulating the hallmarks of cancer cell metabolism. Using these data, we computationally evaluated specifically labeled (13)C glucose and glutamine tracers for their ability to precisely and accurately estimate fluxes in central carbon metabolism. These methods enabled us to identify the optim

SUBMITTER: Metallo CM 

PROVIDER: S-EPMC3026314 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature