Isolation and genetic characteristics of human genotype 1 Japanese encephalitis virus, China, 2009.

ABSTRACT:

Background

Several studies have shown that the predominant genotype of Chinese Japanese encephalitis virus (JEV) is evolving from genotype 3 to genotype 1. However, in recent years, almost all genotype 1 isolates were from mosquitoes, and genotype 1 has been less associated with human disease than genotype 3. This study reports the isolation of human genotype 1 JEV and its genetic characteristics to provide additional insights into human JE pathogens that are currently circulating in China.

Methods and results

In 2009, 31 cerebrospinal fluid samples were collected from patients living in Yunnan and Shanxi provinces and were used to inoculate Aedes albopictus C6/36 cells for virus isolation. The JEV strains were identified using immunofluorescent assays and the reverse transc

SUBMITTER: Zhang JS 

PROVIDER: S-EPMC3026811 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature