An in vitro model for Pelger-Huet anomaly: stable knockdown of lamin B receptor in HL-60 cells.

ABSTRACT: The principal human blood granulocyte (neutrophil) possesses a lobulated and deformable nucleus, important to facilitate rapid egress from blood vessels as these cells migrate to sites of bacterial or fungal infection. This unusual nuclear shape is a product of elevated levels of an integral membrane protein of the nuclear envelope lamin B receptor (LBR) and of decreased amounts of lamin A/C. In humans, a genetic deficiency of LBR produces Pelger-Huët anomaly, resulting in blood neutrophils that exhibit hypolobulated nuclei with redistributed heterochromatin. Structural changes in nuclear architecture occur during granulopoiesis within bone marrow. The exact mechanisms of this nuclear shape change and of heterochromatin redistribution remain largely unknown. As a tool to facilitate analysi

SUBMITTER: Olins AL 

PROVIDER: S-EPMC3027054 | biostudies-literature | 2010 Nov-Dec

REPOSITORIES: biostudies-literature