The adipocyte-expressed forkhead transcription factor Foxc2 regulates metabolism through altered mitochondrial function.

ABSTRACT:

Objective

Previous findings demonstrate that enhanced expression of the forkhead transcription factor Foxc2 in adipose tissue leads to a lean and insulin-sensitive phenotype. These findings prompted us to further investigate the role of Foxc2 in the regulation of genes of fundamental importance for metabolism and mitochondrial function.

Research design and methods

The effects of Foxc2 on expression of genes involved in mitochondriogenesis and mitochondrial function were assessed by quantitative real-time PCR. The potential of a direct transcriptional regulation of regulated genes was tested in promoter assays, and mitochondrial morphology was investigated by electron microscopy. Mitochondrial function was tested by measuring oxygen consumption and extracellular acidification

SUBMITTER: Lidell ME 

PROVIDER: S-EPMC3028341 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature