Canonical protein splicing of a class 1 intein that has a class 3 noncanonical sequence motif.

ABSTRACT: A Thermobifida fusca intein has two characteristics of class 3 inteins: a noncontiguous covariant Trp-Cys-Thr triplet and a Ser flanking its C terminus. However, it has Cys at position one, characteristic of class 1 inteins. Splicing does not require the internal Cys, which may instead coordinate the active site. Therefore, the intein is class 1.

SUBMITTER: Reitter JN 

PROVIDER: S-EPMC3028663 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature