Characterization of avian γδ T-cell subsets after Salmonella enterica serovar Typhimurium infection of chicks.

ABSTRACT: Avian γδ T lymphocytes are frequently found in blood and organs and are assumed to be crucial to the immune defense against Salmonella infections of chicks. To elucidate the so-far-unknown immunological features of subpopulations of avian γδ T cells in the course of infection, day-old chicks were infected orally with Salmonella enterica serovar Typhimurium. Until 11 days after infection, the occurrence as well as transcription of the CD8 antigen and immunologically relevant protein genes of CD8α(-) and CD8α(+high) (CD8αα(+) CD8αβ(+)) γδ cells were analyzed using flow cytometry and quantitative real-time reverse transcription-PCR (RT-PCR) with blood, spleen, thymus, and cecum samples. After infection, an increased percentage of CD8α(+high) γδ T lymphocytes was found in blood, in spleen, and

SUBMITTER: Pieper J 

PROVIDER: S-EPMC3028855 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature